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- Multi-Purpose bio formulation
- Multi-Action Microbial Consortium
Eco Bio-Enzyme Pet odour microbial bacterium groups reveals superior enzyme performance for use in multiple applications.
Eco Bio-Enzyme Pet odour exhibits a broad range of degradation capabilities needed for a multi- purpose product efficacious in maintenance
of drain line and grease traps, improving septic and waste degradation, cleaning and odor control.
Bacteria in its natural environment
- In their natural environment, bacteria produce hundreds of enzymes in response to the organics present in their environment.
- They produce extracellular enzymes that break down proteins, starches, fats, oils, greases and toilet tissue into smaller particles outside the bacterial cell.
- The bacteria then transport the smaller particles across their cell membrane for use as an energy source and for building of new cellular components.
- Since bacteria detect the organics present as potential food and produce specific enzymes to breakdown these organics, it is a very efficient system.
- Many different enzymes are required to completely break down a substrate.
The Eco Bio-Enzyme Pet odour consortium of bacteria produces key extracellular enzymes, including amylase, cellulase, lipase and protease. Drain lines, grease traps, septic systems and surfaces are nutrient rich systems for bacteria. Although many bacteria can utilize above mentioned organics as food sources, it is the bacteria with the most rapid production of these key enzymes that provide the most dramatic effects .
Safety of Eco Bio-Enzyme Pet odour Consortium
Eco Bio-Enzyme Pet odour contains a blend of safe Bacillus microorganisms. Toxicity studies done by an independent laboratory shows that PIERRE @ D BIO ENZYME consortium has no acute oral toxicity, no acute dermal toxicity, and no acute inhalation toxicity at maximal test dose. Acute dermal irritation and acute eye irritation studies classify
Eco Bio-Enzyme Pet odour consortium as non-irritating. Eco Bio-Enzyme Pet odour consortium does not elicit a skin sensitization reaction.
Product Data Sheet
A stable consortium of safe Bacillus spores
Production of multiple enzymes providing a wide range of degradation capabilities
A synergistic blend that works in concert to provide superior performance across multiple applications
Excretion of high levels of amylase, cellulase, lipase and protease enzymes
Ability to work under aerobic and anaerobic conditions
Single product simplicity for multi-application flexibility
Bacteria Counts:- 5.4 X 107 cfu/ml Bacteria Type:- Blend of Bacillus Spores Salmonella:- Not detected
Appearance:- Creamy white
Fragrance:- Pleasantly perfumed
Shelf-life:- One year; maximum loss of 1.0 log at recommended storage conditions
DOSE RATES (Follow dilution rate)
Eco Bio-Enzyme Pet odour can be diluted to the following ratio 1:9 (one part PIERRE @ D BIO ENZYME and 9 parts water) but must be used on the day and any remaining liquid should be discarded
AGRICULTURE WASTE – reduction of high solids/crusting of waste: liquefaction and cleaning
(i.e. cowsheds, piggeries, poultry farms etc.)
Initial Dose Rate
Regular Maintenance Rate
Method of Application
Effluent pits Ponds
1 kg per 10 tones
1 kg/ 20 000 litres
1 kg/ 250 000 litres
Weekly for two
weeks, then 500 g per week
½ kg/20 000 litres per week
Spray over surfaces.
Spray on surface.
Spray over cone.
Eco Bio-Enzyme Pet odour is designed as a bio-technical aid to treatment of organic waste material offering the following advantages: - Liquefaction & reduction of solids, reduction of odor, easier disposal of waste, aids general cleaning of soiled areas, safety in operation of effluent systems, offers a viable alternative to current processing techniques using a bio-technical approach.
How does Eco Bio-Enzyme Pet odour Liquid out-perform these pathogens?
In contrast to the 5mins kill time as per the SABS standard being the quickest time a sanitizer (QAC- based) is supposed to kill the most common hygiene microorganisms, S. aureus, E. coli and P. aeruginosa. From a hygiene point of view, you need to sanitize and continue working, whether it’s cooking, manufacturing or sanitizing toilets or urinals or cleaning hard surfaces etc. nowadays, sanitizer manufactures are heading towards 1-minute kill time, i.e. The quicker the sanitizer kills bugs, the sooner you’ll start with your work – however this is not always required by any authorities.
However, with Bacillus-containing sanitizers, it’s different as these are live bacteria that need to compete with other microorganisms for food sources hence the longer contact times required. The pattern that bacillus uses (as seen in the study) to kill or out compete the others showed to be due to its morphological structure. That is, where it grows, other microorganisms will move away from that site of the food source. Since it has a larger morphological structure than other orgs (competing against) it ‘sort of’ creates a zone of inhibition of growth of other microorganisms (morphological inhibition) hence, we get fewer numbers of other microorganisms surviving but more bacillus.
For this to be most effective It is always important to know the cfu value of any biological product that has been diluted –or is in its concentrated form - this is to know if the product you have diluted has sufficient active ingredient to fulfil the desired application:
The ready to use Eco Bio-Enzyme Pet odour following thee analyses within SAP – shelf life is typically one year.
The cfu count on the diluted product is calculated on a mass balance as follows:- Let a = volume of Eco Bio-Enzyme Pet odour Conc 10 X FF & let a = 1 gallon
Let b = volume of water = 9 gallons Let c = volume of Eco Bio-Enzyme Pet odour
Then a + b = c
& c = 10 gallons
Doing a mass balance on the spore count:- There are 2 x 1012 cfu in 1 gallon Eco Bio-Enzyme Pet odour 10 XFF
& count of spores in c = unknown = z Then 2 x 1012a + 0b = zc
Then [(2 x 1012 x 1)/10] = [(z x 10)/10]
2 x 1011 cfu/gallon of Eco Bio-Enzyme Pet odour ( ) Converting from gallons to litres
2 x 1011 spores
= 5.2 x 1010 cfu/litre of Eco Bio-Enzyme Pet odour (52 billion CFU/litre)
The information contained in this leaflet is to the best of our knowledge, true & accurate, but any recommendations or suggestions
which may be made are without guarantee since the conditions of use are beyond our control. No license or immunity under any patents is granted or implied.
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